human ipsc-derived neural precursor cells (npcs) Search Results


ipsc derivation  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare ipsc derivation
Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 <t>iPSC</t> line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.
Ipsc Derivation, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human schizophrenia patient ipsc line d2–1  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare human schizophrenia patient ipsc line d2–1
Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 <t>iPSC</t> line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.
Human Schizophrenia Patient Ipsc Line D2–1, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pluripotent stem cells ipscs  (ATCC)
99
ATCC pluripotent stem cells ipscs
Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 <t>iPSC</t> line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.
Pluripotent Stem Cells Ipscs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoe4 4 genotype  (Axol Bioscience)
93
Axol Bioscience apoe4 4 genotype
Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 <t>iPSC</t> line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.
Apoe4 4 Genotype, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ipsc-derived hepatocytes  (ReproCELL)
90
ReproCELL human ipsc-derived hepatocytes
Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 <t>iPSC</t> line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.
Human Ipsc Derived Hepatocytes, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ipsc  (Axol Bioscience)
94
Axol Bioscience human ipsc
Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 <t>iPSC</t> line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.
Human Ipsc, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mesenchymal stem cells  (ATCC)
95
ATCC mesenchymal stem cells
(A) Schematic of experimental design to compare transcriptome of HESCs exposed to BM-MSC-1 or BM-MSC-2 relative to control HESCs. (B) Principal component analysis results of the transcriptome from mRNA-seq data of control HESCs and HESCs cocultured with <t>BM-MSCs</t> (HESC+BM-MSC-1 and HESC+BM-MSC-2). (C) Venn diagram illustrating number of differentially expressed genes (DEGs) identified in HESCs exposed to BM-MSC-1 (purple), BM-MSC-2 (green), and number of DEGs common to both BM-MSC exposures (blue overlap). (D) The heat map of all DEGs in control HESCs and HESCs cocultured with BM-MSCs. The X-axis represents the different samples (N=3, each condition) and the Y-axis shows all 10,139 DEGs. The color key from orange to blue indicates the relative gene expression level from high to low. (E) Ingenuity Pathway Analysis (IPA) identified four significant canonical signaling pathways with −log(p-value) > 1.3 in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (F) Four DEGs within the four canonical pathways identified in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (G,H) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with BM-MSCs. The relative expression was compared to 18s rRNA. (G) Expression of CCL2 was significantly increased in HESC+BM-MSC-1 (N=4, *P=0.04) and HESC+BM-MSC-2 (N=3, **P=0.004) compared to control HESC (N=6). (H) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=4) and significantly increased in HESC+BM-MSC-2 (N=3, ***P=0.0002) compared to control HESC (N=6). Expression of HGF was significantly increased in HESC+BM-MSC-2 compared to HESC+BM-MSC-1 (**P= 0.006). Data are expressed as means ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test.
Mesenchymal Stem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human wtc11 ipsc-derived line wtc11-mtagrfpt-lmnb1  (Allen Institute for Cell Science)
90
Allen Institute for Cell Science human wtc11 ipsc-derived line wtc11-mtagrfpt-lmnb1
(A) Schematic of experimental design to compare transcriptome of HESCs exposed to BM-MSC-1 or BM-MSC-2 relative to control HESCs. (B) Principal component analysis results of the transcriptome from mRNA-seq data of control HESCs and HESCs cocultured with <t>BM-MSCs</t> (HESC+BM-MSC-1 and HESC+BM-MSC-2). (C) Venn diagram illustrating number of differentially expressed genes (DEGs) identified in HESCs exposed to BM-MSC-1 (purple), BM-MSC-2 (green), and number of DEGs common to both BM-MSC exposures (blue overlap). (D) The heat map of all DEGs in control HESCs and HESCs cocultured with BM-MSCs. The X-axis represents the different samples (N=3, each condition) and the Y-axis shows all 10,139 DEGs. The color key from orange to blue indicates the relative gene expression level from high to low. (E) Ingenuity Pathway Analysis (IPA) identified four significant canonical signaling pathways with −log(p-value) > 1.3 in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (F) Four DEGs within the four canonical pathways identified in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (G,H) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with BM-MSCs. The relative expression was compared to 18s rRNA. (G) Expression of CCL2 was significantly increased in HESC+BM-MSC-1 (N=4, *P=0.04) and HESC+BM-MSC-2 (N=3, **P=0.004) compared to control HESC (N=6). (H) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=4) and significantly increased in HESC+BM-MSC-2 (N=3, ***P=0.0002) compared to control HESC (N=6). Expression of HGF was significantly increased in HESC+BM-MSC-2 compared to HESC+BM-MSC-1 (**P= 0.006). Data are expressed as means ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test.
Human Wtc11 Ipsc Derived Line Wtc11 Mtagrfpt Lmnb1, supplied by Allen Institute for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wicell penn022i 89 1  (WiCell Research Institute Inc)
94
WiCell Research Institute Inc wicell penn022i 89 1
(A) Schematic of experimental design to compare transcriptome of HESCs exposed to BM-MSC-1 or BM-MSC-2 relative to control HESCs. (B) Principal component analysis results of the transcriptome from mRNA-seq data of control HESCs and HESCs cocultured with <t>BM-MSCs</t> (HESC+BM-MSC-1 and HESC+BM-MSC-2). (C) Venn diagram illustrating number of differentially expressed genes (DEGs) identified in HESCs exposed to BM-MSC-1 (purple), BM-MSC-2 (green), and number of DEGs common to both BM-MSC exposures (blue overlap). (D) The heat map of all DEGs in control HESCs and HESCs cocultured with BM-MSCs. The X-axis represents the different samples (N=3, each condition) and the Y-axis shows all 10,139 DEGs. The color key from orange to blue indicates the relative gene expression level from high to low. (E) Ingenuity Pathway Analysis (IPA) identified four significant canonical signaling pathways with −log(p-value) > 1.3 in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (F) Four DEGs within the four canonical pathways identified in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (G,H) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with BM-MSCs. The relative expression was compared to 18s rRNA. (G) Expression of CCL2 was significantly increased in HESC+BM-MSC-1 (N=4, *P=0.04) and HESC+BM-MSC-2 (N=3, **P=0.004) compared to control HESC (N=6). (H) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=4) and significantly increased in HESC+BM-MSC-2 (N=3, ***P=0.0002) compared to control HESC (N=6). Expression of HGF was significantly increased in HESC+BM-MSC-2 compared to HESC+BM-MSC-1 (**P= 0.006). Data are expressed as means ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test.
Wicell Penn022i 89 1, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ipsc-derived+neural+precursor+cells+(npcs)/PENN022i-89-1/pm41059695-227-24-24
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wicell penn022i 89 1 - by Bioz Stars, 2026-09
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human ipsc-derived opcs tempo-iologo  (Tempo Bioscience)
90
Tempo Bioscience human ipsc-derived opcs tempo-iologo
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Human Ipsc Derived Opcs Tempo Iologo, supplied by Tempo Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ipsc line hipsc line imr90  (WiCell Research Institute Inc)
97
WiCell Research Institute Inc human ipsc line hipsc line imr90
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Human Ipsc Line Hipsc Line Imr90, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ipsc (hipsc) line sc0000020  (RUCDR Infinite Biologics)
90
RUCDR Infinite Biologics human ipsc (hipsc) line sc0000020
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Human Ipsc (Hipsc) Line Sc0000020, supplied by RUCDR Infinite Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 iPSC line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.

Journal: Cell Stem Cell

Article Title: Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by non-integrating plasmid expression

doi: 10.1016/j.stem.2012.01.005

Figure Lengend Snippet: Mononuclear cells from bone marrow (BM) of a healthy adult donor (2426) were separated into CD34+ cells (~1%) and CD34-depleted (CD34−) cells. The CD34+ cells were cultured for 4 days with hematopoietic cytokines before being reprogrammed by episomal vectors (left). The BC1 iPSC line was derived by using a single plasmid pEB-C5 while the BCT1 iPSC line was derived by addition of a second plasmid pEB-Tg. The BM CD34− cells were used to establish cultures of marrow stromal cells (also called mesenchymal stem cells or MSCs) by first selecting adherent cells followed by selective expansion of MSCs for additional 13 days (after primary and first passage). The harvested MSCs after first passage (p1) were used for reprogramming similarly by episomal vectors. Two independent iPSC lines, E1 and E2, were established and expanded for 15 passages (p15). Functional Characterizations of E1 and BCT1 iPSC lines are shown in Supplemental Figures 1 and 2, while characterization of BC1 iPSC line was published previously (Chou et al., 2011). Three expanded and characterized iPSC lines, BC1, BCT1 and E1 (boxed), were also analyzed by whole genome sequencing at a deep length. This was done in pair with the parental somatic cells (also boxed). While S1/S2 samples were sequenced at BGI as one pair, S3/S4 and S5/S6 samples were sequenced at NIH as two pairs.

Article Snippet: Use of anonymous human samples for laboratory research including iPSC derivation was approved by IRB and the Institutional Stem Cell Research Oversight (ISCRO) committee at Johns Hopkins University.

Techniques: Cell Culture, Derivative Assay, Plasmid Preparation, Functional Assay, Sequencing

Summary of sequencing 3 pairs of iPSC lines and their parental somatic cells

Journal: Cell Stem Cell

Article Title: Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by non-integrating plasmid expression

doi: 10.1016/j.stem.2012.01.005

Figure Lengend Snippet: Summary of sequencing 3 pairs of iPSC lines and their parental somatic cells

Article Snippet: Use of anonymous human samples for laboratory research including iPSC derivation was approved by IRB and the Institutional Stem Cell Research Oversight (ISCRO) committee at Johns Hopkins University.

Techniques: Sequencing, Plasmid Preparation

(A) Schematic of experimental design to compare transcriptome of HESCs exposed to BM-MSC-1 or BM-MSC-2 relative to control HESCs. (B) Principal component analysis results of the transcriptome from mRNA-seq data of control HESCs and HESCs cocultured with BM-MSCs (HESC+BM-MSC-1 and HESC+BM-MSC-2). (C) Venn diagram illustrating number of differentially expressed genes (DEGs) identified in HESCs exposed to BM-MSC-1 (purple), BM-MSC-2 (green), and number of DEGs common to both BM-MSC exposures (blue overlap). (D) The heat map of all DEGs in control HESCs and HESCs cocultured with BM-MSCs. The X-axis represents the different samples (N=3, each condition) and the Y-axis shows all 10,139 DEGs. The color key from orange to blue indicates the relative gene expression level from high to low. (E) Ingenuity Pathway Analysis (IPA) identified four significant canonical signaling pathways with −log(p-value) > 1.3 in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (F) Four DEGs within the four canonical pathways identified in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (G,H) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with BM-MSCs. The relative expression was compared to 18s rRNA. (G) Expression of CCL2 was significantly increased in HESC+BM-MSC-1 (N=4, *P=0.04) and HESC+BM-MSC-2 (N=3, **P=0.004) compared to control HESC (N=6). (H) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=4) and significantly increased in HESC+BM-MSC-2 (N=3, ***P=0.0002) compared to control HESC (N=6). Expression of HGF was significantly increased in HESC+BM-MSC-2 compared to HESC+BM-MSC-1 (**P= 0.006). Data are expressed as means ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test.

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: (A) Schematic of experimental design to compare transcriptome of HESCs exposed to BM-MSC-1 or BM-MSC-2 relative to control HESCs. (B) Principal component analysis results of the transcriptome from mRNA-seq data of control HESCs and HESCs cocultured with BM-MSCs (HESC+BM-MSC-1 and HESC+BM-MSC-2). (C) Venn diagram illustrating number of differentially expressed genes (DEGs) identified in HESCs exposed to BM-MSC-1 (purple), BM-MSC-2 (green), and number of DEGs common to both BM-MSC exposures (blue overlap). (D) The heat map of all DEGs in control HESCs and HESCs cocultured with BM-MSCs. The X-axis represents the different samples (N=3, each condition) and the Y-axis shows all 10,139 DEGs. The color key from orange to blue indicates the relative gene expression level from high to low. (E) Ingenuity Pathway Analysis (IPA) identified four significant canonical signaling pathways with −log(p-value) > 1.3 in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (F) Four DEGs within the four canonical pathways identified in HESCs cocultured with BM-MSC-1 and BM-MSC-2. (G,H) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with BM-MSCs. The relative expression was compared to 18s rRNA. (G) Expression of CCL2 was significantly increased in HESC+BM-MSC-1 (N=4, *P=0.04) and HESC+BM-MSC-2 (N=3, **P=0.004) compared to control HESC (N=6). (H) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=4) and significantly increased in HESC+BM-MSC-2 (N=3, ***P=0.0002) compared to control HESC (N=6). Expression of HGF was significantly increased in HESC+BM-MSC-2 compared to HESC+BM-MSC-1 (**P= 0.006). Data are expressed as means ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test.

Article Snippet: Human BM-derived mesenchymal stem cells (ATCC) were derived from a 35-year-old Hispanic female donor.

Techniques: Control, Gene Expression, Protein-Protein interactions, Quantitative RT-PCR, Expressing

(A-D) Flow cytometric analysis was performed on (A) BM-MSC-1, (B) BM-MSC-2, (C) BM-MSC-3 and (D) BM-MSC-4. MSC surface markers CD44, CD73, CD29, and CD90 were highly expressed, whereas expression of hematopoietic stem cell marker CD45 was not detected. The percentages of cells expressing each surface marker are indicated. (E-H) Following culture of BM-MSCs in adipocyte differentiation media for 21 days, Oil Red O staining was used to detect adipocytes. Lipid droplets appear red, and nuclei appear blue. (I) BM-MSC-1 cultured in control MSC media for 21d and stained with Oil Red O. (J-M) Following exposure of BM-MSCs to osteoblast differentiation media for 30 days, von Kossa staining was used to identify osteoblasts. Bone nodules containing calcium mineral stain black. (N) BM-MSC-1 cultured in control MSC media for 30d and stained with von Kossa stain. Scale bars: 50 mm.

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: (A-D) Flow cytometric analysis was performed on (A) BM-MSC-1, (B) BM-MSC-2, (C) BM-MSC-3 and (D) BM-MSC-4. MSC surface markers CD44, CD73, CD29, and CD90 were highly expressed, whereas expression of hematopoietic stem cell marker CD45 was not detected. The percentages of cells expressing each surface marker are indicated. (E-H) Following culture of BM-MSCs in adipocyte differentiation media for 21 days, Oil Red O staining was used to detect adipocytes. Lipid droplets appear red, and nuclei appear blue. (I) BM-MSC-1 cultured in control MSC media for 21d and stained with Oil Red O. (J-M) Following exposure of BM-MSCs to osteoblast differentiation media for 30 days, von Kossa staining was used to identify osteoblasts. Bone nodules containing calcium mineral stain black. (N) BM-MSC-1 cultured in control MSC media for 30d and stained with von Kossa stain. Scale bars: 50 mm.

Article Snippet: Human BM-derived mesenchymal stem cells (ATCC) were derived from a 35-year-old Hispanic female donor.

Techniques: Expressing, Marker, Staining, Cell Culture, Control

HESC transwell migration and invasion through Matrigel were assessed after indirect coculture with BM-MSCs for 24h. (A) Diagram of the coculture system of HESCs with BM-MSCs used to assess HESC migration and invasion. (B) Representative images of H&E-stained HESCs that migrated or invaded after 24h coculture. Scale bars 100μM. (C) Transwell migration was significantly increased by coculture with BM-MSC-1 (***P=0.0009) and BM-MSC-2 (***P=0.0004) compared to control HESCs. (D) Transwell invasion through Matrigel was significantly increased by coculture with BM-MSC-1 (*P =0.02) and BM-MSC-2 (****P< 0.0001) compared to control HESC. Data are expressed as means (of N=3–6) ± SEM. Statistical analysis was performed with an unpaired t test followed by Welch’s correction.

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: HESC transwell migration and invasion through Matrigel were assessed after indirect coculture with BM-MSCs for 24h. (A) Diagram of the coculture system of HESCs with BM-MSCs used to assess HESC migration and invasion. (B) Representative images of H&E-stained HESCs that migrated or invaded after 24h coculture. Scale bars 100μM. (C) Transwell migration was significantly increased by coculture with BM-MSC-1 (***P=0.0009) and BM-MSC-2 (***P=0.0004) compared to control HESCs. (D) Transwell invasion through Matrigel was significantly increased by coculture with BM-MSC-1 (*P =0.02) and BM-MSC-2 (****P< 0.0001) compared to control HESC. Data are expressed as means (of N=3–6) ± SEM. Statistical analysis was performed with an unpaired t test followed by Welch’s correction.

Article Snippet: Human BM-derived mesenchymal stem cells (ATCC) were derived from a 35-year-old Hispanic female donor.

Techniques: Migration, Staining, Control

(A, B) Flow cytometric analysis was performed on UC-MSC-1 and UC-MSC-2. MSC surface markers CD44, CD73, CD29, and CD90 were highly expressed, whereas expression of hematopoietic stem cell marker CD45 was not detected. The percentages of cells expressing each surface marker are indicated. (C, D) Following culture of UC-MSCs in adipocyte differentiation media for 21 days, Oil Red O staining was used to detect adipocytes. Lipid droplets appear red, and nuclei appear blue. (E) UC-MSC-1 cultured in control MSC media for 21d and stained with Oil Red O. (F, G) Following exposure of UC-MSCs to osteoblast differentiation media for 30 days, von Kossa staining was used to identify osteoblasts. Bone nodules containing calcium mineral stain black. (H) U-MSC-1 cultured in control MSC media for 30d and stained with von Kossa stain. Scale bars: 50 mm.

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: (A, B) Flow cytometric analysis was performed on UC-MSC-1 and UC-MSC-2. MSC surface markers CD44, CD73, CD29, and CD90 were highly expressed, whereas expression of hematopoietic stem cell marker CD45 was not detected. The percentages of cells expressing each surface marker are indicated. (C, D) Following culture of UC-MSCs in adipocyte differentiation media for 21 days, Oil Red O staining was used to detect adipocytes. Lipid droplets appear red, and nuclei appear blue. (E) UC-MSC-1 cultured in control MSC media for 21d and stained with Oil Red O. (F, G) Following exposure of UC-MSCs to osteoblast differentiation media for 30 days, von Kossa staining was used to identify osteoblasts. Bone nodules containing calcium mineral stain black. (H) U-MSC-1 cultured in control MSC media for 30d and stained with von Kossa stain. Scale bars: 50 mm.

Article Snippet: Human BM-derived mesenchymal stem cells (ATCC) were derived from a 35-year-old Hispanic female donor.

Techniques: Expressing, Marker, Staining, Cell Culture, Control

HESCs were analyzed for cellular proliferation using Ki67 after 24h indirect coculture with BM-MSCs. (A) Diagram of the coculture system of HESCs with BM-MSCs used to assess HESC proliferation. (B) Representative IF images of HESCs stained with Ki67. Scale bars: 100μM. (C) Coculture with BM-MSC-1 significantly increased HESC proliferation (*P=0.02). HESC proliferation was unchanged with exposure to BM-MSC-2.

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: HESCs were analyzed for cellular proliferation using Ki67 after 24h indirect coculture with BM-MSCs. (A) Diagram of the coculture system of HESCs with BM-MSCs used to assess HESC proliferation. (B) Representative IF images of HESCs stained with Ki67. Scale bars: 100μM. (C) Coculture with BM-MSC-1 significantly increased HESC proliferation (*P=0.02). HESC proliferation was unchanged with exposure to BM-MSC-2.

Article Snippet: Human BM-derived mesenchymal stem cells (ATCC) were derived from a 35-year-old Hispanic female donor.

Techniques: Staining

(A) After coculture with UC-MSCs for 24h, HESCs were analyzed for cellular proliferation using Ki67. HESC proliferation was unchanged with exposure UC-MSC-1. Coculture with UC-MSC-2 significantly increased HESC proliferation (*P=0.01). (B) HESC transwell migration was assessed after coculture with UC-MSCs for 24h. Transwell migration was significantly increased by coculture with UC-MSC-1 (N=5, ***P=0.0004) and UC-MSC-2 (N=3, *P=0.01) compared to control HESC (N=5). (C) Transwell invasion through Matrigel was significantly increased by coculture with UC-MSC-1 (N=6, ****P< 0.0001) and UC-MSC-2 (N=3, ***P=0.0008) compared to control HESC (N=6) (D, E) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with UC-MSCs. The relative expression was compared to 18s rRNA. (D) Expression of CCL2 was unchanged in HESC+UC-MSC-1 (N=7) and significantly increased in HESC+UC-MSC-2 (N=7, **P=0.005) compared to control HESC (N=6). (E) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=7) and significantly increased in HESC+UC-MSC-2 (N=7), **P=0.008) compared to control HESC (N=6). Data are expressed as means (of N=3–6) ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test (A, D, E) or an unpaired t test followed by Welch’s correction (B, C).

Journal: Reproduction (Cambridge, England)

Article Title: Mesenchymal Stem Cell Secretome Alters Gene Expression and Upregulates Motility of Human Endometrial Stromal Cells

doi: 10.1530/REP-22-0485

Figure Lengend Snippet: (A) After coculture with UC-MSCs for 24h, HESCs were analyzed for cellular proliferation using Ki67. HESC proliferation was unchanged with exposure UC-MSC-1. Coculture with UC-MSC-2 significantly increased HESC proliferation (*P=0.01). (B) HESC transwell migration was assessed after coculture with UC-MSCs for 24h. Transwell migration was significantly increased by coculture with UC-MSC-1 (N=5, ***P=0.0004) and UC-MSC-2 (N=3, *P=0.01) compared to control HESC (N=5). (C) Transwell invasion through Matrigel was significantly increased by coculture with UC-MSC-1 (N=6, ****P< 0.0001) and UC-MSC-2 (N=3, ***P=0.0008) compared to control HESC (N=6) (D, E) RT-qPCR was used to determine expression of CCL2 and HGF in HESCs cocultured with UC-MSCs. The relative expression was compared to 18s rRNA. (D) Expression of CCL2 was unchanged in HESC+UC-MSC-1 (N=7) and significantly increased in HESC+UC-MSC-2 (N=7, **P=0.005) compared to control HESC (N=6). (E) Expression of HGF was unchanged in HESC+BM-MSC-1 (N=7) and significantly increased in HESC+UC-MSC-2 (N=7), **P=0.008) compared to control HESC (N=6). Data are expressed as means (of N=3–6) ± SEM. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparisons test (A, D, E) or an unpaired t test followed by Welch’s correction (B, C).

Article Snippet: Human BM-derived mesenchymal stem cells (ATCC) were derived from a 35-year-old Hispanic female donor.

Techniques: Migration, Control, Quantitative RT-PCR, Expressing

Table of materials

Journal: Journal of visualized experiments : JoVE

Article Title: Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-oligodendrocyte Interactions

doi: 10.3791/61778

Figure Lengend Snippet: Table of materials

Article Snippet: Tempo-iOlogo: Human iPSC-derived OPCs , Tempo BioScience , SKU102.

Techniques: